Abstract:
To explore the potential value of polyphenols in edible dock, in this study, single-factor and response surface experiments were carried out to optimize the extraction of polyphenols from edible dock, and macroporous adsorption resin and polyamide were used to enrich and refine them. The results showed that when the extraction temperature was 39 ℃, the ratio of solid to liquid was 1∶33 g·mL
-1, and the volume fraction of ethanol was 78%, the content of polyphenols was the highest, which could reach (22.73±2.73) mg/g. After freeze-drying, the polyphenol purity of the crude extract was 7.20%±0.51%. By screening of macroporous resins of different polarity, it was found that AB-8 resin had the best adsorption and desorption effect. After the enrichment of polyphenols by this resin, the purity of polyphenols reached 34.28%±2.86%, the polyphenols was further refined and eluted with 40% ethanol. The highest purity of polyphenols was 53.57%±4.31%. Compared with the crude extract, the purity of polyphenols was 7.3 times higher. Further analysis by high performance liquid chromatography (HPLC) showed that the macroporous adsorbent resin and polyamide had good enrichment, refining and crude separation effects on the polyphenols of the edible dock. In this study, the extraction process of polyphenols was optimized, and the refining of polyphenols was realized effectively, which provided a reference for expanding the application of the edible dock.