Abstract: For high value-added development and utilization of coconut oil, a product enriched with medium chain triglyceride (MCT) was synthesized by H₂SO₄-catalyzed acidolysis of coconut oil with caprylic acid (Cy). Effects of reaction time, reaction temperature, molar ratio of Cy to coconut oil and catalyst amount on the product were investigated through single factor experiments and then optimized by the Box-Behnken center combination experimental design principle, taking medium chain fatty acid (MCFA) content in the products as the response value. The optimum reaction conditions were shown as follows: reaction time of 3 h, substrate molar ratio of 6∶1, catalyst amount of 5% and reaction temperature of 110 ℃. Under these conditions, the MCFA content in the product were 82.13%, which was nearly consistent with the predicted value. In addition, contents of Cy, sn-2 Cy and sn-2 MCFA in the product were 56.42%, 43.17% and 76.17%, respectively. Moreover, content of MCT in the product was quantified to be higher than 50.87% by high performance liquid chromatography. The results could provide references for deep development and utilization of coconut oil.