Abstract: Ningxia 843 japonica rice starch was used as raw material to study the optimum process for the preparation of porous starch by single factor and orthogonal design. The main physicochemical properties and adsorption applications of the prepared porous starch were investigated and compared. The optimum conditions were: reaction temperature 37 ℃, reaction time 14 h, enzyme amount 35%, pH 4.5. Results of tungsten filament scanning electron microscopy showed that the porous starch had a hollow structure and slightly rough surface with holes, and the starch particles were basically intact without collapses. The specific volume, expansion rate, light transmittance and oil absorbency of the porous rice starch prepared under the optimum conditions were 0.401 g/mL, 15.2%, 9.6% and 99.8%, increased by 19.7%, 34.5%, 12.9% and 45.3% in comparision with raw rice starch, respectively. Copper ion adsorption of the original porous starch from japonica rice increased by 51.9% in comparision with raw rice starch, and that of porous amino starch increased by 45.2% and 120.5% compared with the original porous starch and raw rice starch. Compared with raw starch, the specific volume, expansion rate, light transmittance and oil absorbency of porous starch increased. The copper ion absorption capacity of porous starch was enhanced obviously after amino modification.