Abstract: To improve the lipid solubility, stability, bioavailability and slow-release effect of amygdalin, amygdalin-loaded liposomes were prepared by reverse evaporation method. Based on single factor and orthogonal experiments, the preparation conditions were optimized. The physicochemical properties, storage stability, and in vitro release characteristics of the liposomes were also studied. The optimum conditions for preparing amygdalin-loaded liposomes were as follows: the mass ratio of phospholipids to cholesterol was 4∶1, the volume ratio of organic phase to water phase was 2∶1, pH value of phosphate buffer solution was 6.6, and the mass ratio of amygdalin to phospholipids was 5%. Under the optimum conditions, the encapsulation efficiency of amygdalin-loaded liposomes was (33.42±1.76)%. The average vesicle size of amygdalin-loaded liposomes was (189.7±1.3) nm with the polydispersity index of 0.16±0.02, and the zeta potential was (-31.20±2.41) mV.The morphology of amygdalin-loaded liposomes was observed by transmission electron microscope. It showed that amygdalin-loaded liposomes vesicles were spherical and distributed uniformly. In addition, the results indicated that amygdalin-loaded liposomes were relatively stable during the storage period and could effectively inhibit the leakage, aggregations, and membrane oxidation of amygdalin especially at low temperature. This study also suggested that the release rate of amygdalin could be reduced by encapsulating with liposomes thereby achieving better slow-release characteristics.