Abstract: To understand the relationship between the physiological indexes and aging time and provide data support for the selection of sensitive indexes in the aging process of wheat, this study determined the following changes:wheat conductivity, germination rate, viability, the activities of peroxidase and catalase, falling number, and malondialdehyde content of medium and high-gluten wheat during artificial accelerated aging(40 ℃, RH 75%). The results showed that, with the extension of accelerated aging time, the electrical conductivity of medium and high-gluten wheat increased by 181% and 133% respectively, the germination rate and viability decreased by 100%. The catalase activities of medium and high-gluten wheat decreased by 89.46% and 83.36%, respectively. The maximum decrease of peroxidase activity was detected in 4-5 months, for the medium gluten wheat, the decrease was 90.79%, and for the high gluten wheat, the decrease was 73.94%. The falling number showed an upward trend, the growth rate of the medium-gluten wheat was 88.94%, and that of the high-gluten wheat was 87.59%. The content of malondialdehyde also increased by 107.38%and 94.65% for the medium and high-gluten wheat, respectively.Descriptive statistical analysis and variance analysis were carried out on the physiological indexes of medium and high-gluten wheat in different aging stages, and the results showed that the differences of each physiological index during aging were significant (P< 0.05) with high variable coefficient, indicating that all these indexes could be used to evaluate the aging of wheat, and the most prominent indexes (germination rate, viability, and the activities of peroxidase and catalase) could be further screened.