Abstract: This study established a microwave-assisted complex enzymatic method for the extraction of polysaccharide from Schisandra chinensis Baill. Taking the polysaccharide yield as the evaluation index, response surface methodology and orthogonal test were used to optimize the composition of complex enzymes and the extraction process of the current microwave-assisted complex enzymatic method. The optimal complex enzyme was composed of cellulase, papain and pectinase with mass ratio of 1∶2∶2; the optimum extraction technology were as follows: microwave irradiation time of 10 min, pH 4.0, enzymatic hydrolysis time 1.2 h at 45 ℃. Under these optimal conditions, the extraction yield of polysaccharides reached 14.87%, which was higher than that of traditional heat reflux, microwave-assisted heat reflux, ultrasonic-assisted method, and single enzyme method. The approach proposed has the advantages of simple operation, short time consuming, low extraction temperature, and high polysaccharide yield, which provided a theoretical basis and data support for the industrial production of polysaccharide from Schisandra chinensis (Turcz.) Baill.