Abstract: Tribolium castaneum (Herbst) is a harmful stored grain pest in the world. It is important to explore the response of red flour beetle upon chemical substances. The volatile odors released by plants are closely related to insect's living behaviors such as localization, feeding, and reproduction. Electroantennogram (EAG) and four-arm-olfactometer are commonly used to identify the effects of plant volatiles on insects. The activity of the stimulant to insects can be judged by the presence and size of the EAG, and the active substances can be screened out quickly and accurately; the behavioral responses of insects to stimuli can be observed directly by using four-arm-olfactometer. The two methods are of great significance for comprehensive judgment of the effects of irritants on insects. Wheat flour has a certain attraction effect on red flour beetle, and it is the most common host of the insect. There are many kinds of volatiles from wheat flour, but not all of them are attractive to red flour beetle. In order to explore the tendency of red flour beetle to different components of wheat flour volatiles, the EAG and behavioral responses of adult red flour beetles to 14 volatile components of wheat flour were tested by the above two techniques and methods. The 14 wheat flour volatiles were propanol, valeraldehyde, 2-methyl-3-buten-1-ol, 4-cyclopentene-1, 3-diol, cyclopentacycloheptene, 2-pentylfuran, 3-octen-2-one, 2-octenal, nonanal, 3-ethyl ortho-xylene, dodecane, decanal, tetradecane, hexadecane. The results showed that the EAG response value did not change significantly with the increase of the concentration of several hydrocarbon volatiles in the tested concentration range, and the EAG value ranged from 0.1mV to 0.3 mV; the maximum EAG responses of the red flour beetle to 2-octene aldehyde and 2-methyl-3-butene-1-ol appeared at 5 μg/μL, with EAG values of 0.89 mV and 0.69 mV;the maximum EAG responses to 3-octene-2-one and 4-cyclopentene-1, 3-diol appeared at 0.5 μg/μL, with EAG values of 0.66 mV and 0.72 mV. In the behavioral test, pentanal(0.5, 50 μg/μL), nonanal(50 μg/μL)and 2-octenal(0.5 μg/μL)have shown a significant attraction to the red flour beetle(P< 0.05), with the highest trapping rates of 36.67%, 37.77% and 35.57%, respectively. The other wheat flour volatiles did not show obvious attractions or repellent effects on the adult insects. Pentanal, nonanal, 2-octenal and some other substances that can induce high EAG responses of the red flour beetle may play an important role in the attractiveness of wheat flour volatiles to the red flour beetle. It is necessary to verify the behavior response of several volatile compounds with strong EAG and behavior response in the actual environment.