糊粉层粉对面团蛋白质聚集和挂面品质的影响

    Effect of aleurone flour on protein aggregation characteristics in dough and quality of dried noodles

    • 摘要: 为了探究富含营养的糊粉层粉对面团蛋白分子聚集以及挂面品质的影响,采用不同配比的糊粉层粉与面粉混合粉作为原料制作面团和挂面。结果表明:随着糊粉层粉添加量的增加,挂面的吸水率先降低后升高,蒸煮损失率显著增加;与对照组相比,煮后的挂面随着糊粉层粉添加量的增加延伸性显著降低,弹性降低,硬度和咀嚼性增加;随着糊粉层粉添加量的增加,挂面色泽的L*显著降低;糊粉层粉的添加对挂面的感官品质有显著影响;随着糊粉层粉添加量的增加,面团麦醇溶蛋白逐渐发生聚集,麦谷蛋白发生解聚,面团游离巯基的含量显著增加。相比没有添加糊粉层粉的挂面,糊粉层粉加入后挂面品质下降,主要是面团蛋白质分子量的改变和聚集对其产生影响。

       

      Abstract: In order to study the effect of the addition of nutritious aleurone flour on protein aggregation characteristics in dough and the effect of different proportions of aleurone flour on the quality of dried noodles, different aleurone flour with varied ratios (0/100, 10/90, 20/80, 30/70, 40/60, 50/50) were used as the materials to make dried noodles. The results showed that the water absorption of aleurone dried noodles first decreased and then increased with the optimal cooking time, and the cooking lose rate increased significantly with the increase of the aleurone flour addition. In addition, when the addition amount of aleurone flour was more than 30/70, more noodles were broken with the increase of the addition amount. The ultrastructure of the dried noodles showed that the wall section was embedded with a large number of fiber structures. Tensile properties and total texture properties of cooked noodles were measured with texture profile analysis. Compared with the dried noodles without aleurone flour, with the increase of the aleurone flour addition amount, the breaking distance and elasticity significantly reduced, the hardness and chewiness increased. The dried noodles were ground into powder and screened for color determination. Compared with normal noodles, with the increase of the addition amount of aleurone flour, the L* value decreased significantly, and the color of the dried noodles gradually turned yellow and dark. Hence, the addition of aleurone flour has a significant impact on the sensory quality of the dried noodles. The protein extractability of dough was determined by high performance liquid chromatography and showed that with the addition of aleurone flour, the gliadin aggregated gradually and the glutenin depolymerized. Moreover, free SH content in dough increased significantly. The change of protein extractability reflected the regular pattern of protein aggregation in dough. The protein aggregation characteristics in dough affects the quality of dried noodles. Compared with the noodles without aleurone flour, the quality of the aleurone noodles decreased. The change of protein aggregation in dough can be used to explain the change of the quality of the dried noodles with the increase of the aleurone flour.

       

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