Abstract: With the approval of peony seed oil as a new resource food, it has attracted great interests. Peony seed oil is rich in linolenic acid, linoleic acid, and other unsaturated fatty acids, which plays important roles in improving the functions of cardiovascular and cerebrovascular, regulating the immunity, preventing tumor and inflammatory. Therefore, peony seed oil has important development and utilization values. In this review, extraction technologies of peony seed oil were introduced, such as mechanical pressing, solvent extraction, supercritical CO₂ extraction, hydroenzymatic extraction, subcritical low temperature extraction, ultrasonic assisted extraction, and microwave assisted extraction. This review will provide a reference for further development and utilization of peony seed oil.