Abstract: In order to selectively enrich and separate trace aflatoxin in grains, a solid-phase extraction filler with low price, stable performance and strong enrichment ability was developed. Quercetin, a structural analog of aflatoxin, was used as an alternative template, acrylamide as a monomer, ethylene glycol dimethacrylate as a crosslinking agent and azodiisobutyronitrile as an initiator, quercetin molecularly imprinted polymer was prepared by precipitation polymerization.The optimum reaction conditions were determined by single factor and orthogonal test:the ratio of template:monomer:crosslinking agent (molar ratio) was 1:6:20, the amount of initiator (percentage of total mass of monomer and crosslinking agent) was 3%, and the reaction time was 5 h.The polymers were characterized by an infrared spectrometer, a scanning electron microscope and a particle size distribution instrument. The adsorption performance of the polymers was measured by an ultraviolet-visible spectrophotometer. The results showed that the adsorption capacity of imprinted polymers can reach 12.76 μg/mg, and the adsorption capacity can reach the adsorption equilibrium within 10 minutes. In addition, the adsorption performance of imprinted polymers was significantly better than that of non-imprinted polymers. Using quercetin molecularly imprinted polymer as the adsorbent, a solid phase extraction column was prepared for the pretreatment of aflatoxin in wheat. Combined with high performance liquid chromatography, the method was established. The performance parameters such as detection limit, quantitative limit, linear range, accuracy and precision of the method were evaluated. The method can be used for the separation, purification and analysis of trace aflatoxin in grains, as well, provides a new method for monitoring grain pollution.