锈赤扁谷盗磷化氢抗性的不同治理方法及效果

赵艳艳; 鲁玉杰; 张蒙

doi:10.16433/j.1673-2383.2022.02.015

锈赤扁谷盗磷化氢抗性的不同治理方法及效果

河南工业大学粮油食品学院, 粮食储藏与安全教育部工程研究中心, 粮食储运国家工程实验室, 河南郑州 450001

基金项目:

河南省自然科学基金项目(202300410105)；河南省高等学校重点科研项目(20A210008)；河南工业大学高层次人才科研基金项目(2018BS069)

详细信息

作者简介:
赵艳艳(1991-),女,河南开封人,硕士研究生,研究方向为储藏物害虫综合治理

通讯作者:
鲁玉杰,教授,博导,E-mail:luyujie1971@163.com

中图分类号: S379.5
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出版历程
- 收稿日期: 2021-11-14
- 网络出版日期: 2022-10-12
- 刊出日期: 2022-10-12

Different control methods and their effects on phosphine resistance in Cryptolestes ferrugineus (Stephens)

College of Food Science and Engineering, Engineering Research Center of Grain Storage and Security of Ministry of Education, Grain Storage and Logistics National Engineering Laboratory, Henan University of Technology, Zhengzhou 450001, China

摘要

摘要: 锈赤扁谷盗已经对磷化氢产生了严重的抗药性，研究其抗性治理的方法对于防治害虫具有重要意义。采用延长磷化氢熏蒸时间(延长时间为连续7 d)和3种防护剂(甲基嘧啶磷、溴氰·甲嘧磷和马拉硫磷)对磷化氢抗性的锈赤扁谷盗进行防治效果的研究。结果表明：延长磷化氢熏蒸时间对锈赤扁谷盗的杀虫效果显著，磷化氢质量浓度1.38 mg/L时，熏蒸2、3、4、5、6、7 d，锈赤扁谷盗在2～5 d时死亡率不足50%，在6 d时死亡率为76%，在7 d时死亡率为94%，死亡率增加显著，说明锈赤扁谷盗表现了极高的磷化氢抗性，对其延长磷化氢熏蒸时间比提高浓度防治更可行；3种防护剂中甲基嘧啶磷和溴氰·甲嘧磷比马拉硫磷防治效果好；分别使用2、4、6、8、10 μg/cm²的甲基嘧啶磷延长触杀时间，6、10 μg/m²甲基嘧啶磷分别处理48、10 h，锈赤扁谷盗的死亡率均达到了100%。因此，可适当延长磷化氢的熏蒸时间或防护剂的处理时间，适当增加防护剂的剂量均能显著提高锈赤扁谷盗的死亡率，既能达到害虫防治效果又能延缓抗药性。
- 锈赤扁谷盗 /
- 磷化氢 /
- 防护剂 /
- 甲基嘧啶磷 /
- 马拉硫磷 /
- 溴氰· /
- 甲嘧磷
Abstract: Cryptodestes ferrugineus (Stephens) is one of the world's stored grain pest and one of the main stored grain pests in China. At present, phosphine fumigation is the most commonly used method for the prevention and control of C. ferrugineus. In recent years, the resistance of C. ferrugineus against phosphine has become more and more serious. This problem has become the most difficult challenge for application phosphine and pest control in stored grain. In order to effectively prevent and control the damage caused by C. ferrugineus in grain storage, it is of great significance and urgent to study the resistance management methods for pest control. In this paper, the effects of prolonging the fumigation time of phosphine and three protective agents on the control of C. ferrugineus resistant to phosphine were studied. The resistance level of the adult of C. ferrugineus against phosphine was measured by FAO method, the toxicity of pirimiphos-methyl, malathion and deltamethrim-pirimipins-methyl against adults of C. ferrugineus was determined by filter paper method. Contact killing effect of pyrimidine methyl on the adult of C. ferrugineus was evaluated. The results showed that prolonging the fumigation time of phosphine had a significant insecticidal effect on C. ferrugineus. When the concentration of phosphine was 1.38 mg/L, after the fumigation time for 2, 3, 4, 5, 6 and 7 days, the mortality rate was less than 50% in 2 to 5 days, 76% in 6 days and 94% in 7 days, and the mortality rate increased with the fumigant time significantly. When the concentration of phosphine was 13.8 mg/L for 20 h, the survival rate of C. ferrugineus was 98%. The results indicated that C. ferrugineus showed extremely high phosphine resistance, and it was more feasible to prolong the fumigation time of phosphine than to increase the concentration of phosphine. The LD₅₀ of three kinds of grain protectant pirimiphos-methyl, malathion and deltamethrim-pirimipins-methyl for 24 hours were 7.519, 26.914 and 6.071 μg/cm², respectively. The LD₅₀ for 48 hours were 5.031, 19.401 and 3.796 μg/cm², respectively. After 72 hours treatment, the LD₅₀ were 3.438, 13.646 and 2.936 μg/cm², respectively. These results indicated that among the three grain protectants, pirimiphos-methyl and deltamethrim-pirimipins-methyl had better effect than malathion. The mortality rate of C. ferrugineus reached 100% after being treated with pirimiphos-methyl 6 μg/cm² for 48 hours and 10 μg/cm² for 10 hours. The results showed that prolonging the treatment time and increasing the dosage of the grain protectant significantly increased the mortality rate of C. ferrugineus. Therefore, the dosage of protective agent can be appropriately reduced or the treatment time can be prolonged, which can not only achieve the pest control effect but also delay the development of resistance.
- Cryptolestes ferrugineus /
- phosphine /
- grain protectant /
- pirimiphos-methyl /
- malathion /
- deltamethrim-pirimipins-methyl

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