Abstract: White rot fungi is one of the known microorganisms that can efficiently degrade lignocellulose. In order to predict and screen the genes related to the degradation of wheat bran fiber by White rot fungi, A. polytricha 5.584 was cultured in liquid culture environment with wheat bran fiber and glucose as single carbon source, the transcriptome was sequenced by high-throughput sequencing technology, and the transcripts were compared and analyzed by GO and other databases. A total of 38. 18 Gb clean data were obtained from the sequencing results of BGI-500 high-throughput sequencing platform. After recombination and de-redundancy, a total of 31 531 unigenes were obtained. The total length, average total length, N50, and its GC concentration were 57226025 bp, 1814 bp, 2537 bp, and 52.42%, respectively. 24404 CDS were detected by transcoder, and 1989 SSRs were also detected to be scattered in 1536 unigenes. The comparison efficiency between clean reads of each sample and the reference genome was 86.29%-89.49%. The FPKM density distribution results showed that the grouping was reasonable and there were differences between groups. By using DEGseq to screen differentially expressed genes and comparing them with the seven functional database systems, 8214 up-regulated genes and 3882 down regulated genes were obtained, of which 8 638 were annotated into the GO database, and they were marked in 42 secondary entries of the three main functional classifications. The number of different gene expression groups annotated to the secondary function of "biological process" function "cell metabolism step (GO:0008152)" was 1 088. There were 1 111 differential genes annotated to the secondary function groups "membrane (GO:0016020)" of "cell component" function. There were 1 818 differential genes annotated to the secondary function "catalytic activity (GO:0003824)" of "gene molecular structure and function", and 4 310 differentially expressed genes were annotated into KEGG database. Finally, the protein interaction of differentially expressed genes was analyzed by using diamond software, and the network relationship with interaction relationship score greater than or equal to 300 was selected for mapping. Five key genes related to the degradation of wheat bran fiber were obtained include CL16. Contig1_All、CL1024. Contig1_All、CL2915. Contig1_All、CL710. Contig10_All and CL3860. Contig3_All. This study provides a reference for the in-depth study of the degradation mechanism of White rot fungi on wheat bran dietary fiber.