Abstract: Newly developed rapid detection methods of the specific food-borne pathogen based on molecular biology and immunology has the benefit to shorten the experimental procedure time and improve detection efficiency since 2000. However, a scientific and systematic comparison study must be conducted to confirm the method equivalence between the newly developed methods and the traditional standard methods. Meanwhile, different standard methods from different countries for same testing purpose are expected to be evaluated for their method equivalence. The equivalence evaluation results between different test methods can provide efficient data to scientifically support products release and compliance inspection for food imports and exports cross board or regions. This article describes the progress of micro method equivalence study based on the specific food-borne pathogen, summarizes and discusses the advantage and challenge of method equivalence study.