Abstract: With the development of economy, food safety has become a focus of people's attention. In recent decades, due to the many advantages and high value of peanut oil, the global production of peanut oil has been increasing. However, peanuts are easily infected with aflatoxin (AFT) during production and transportation, and since AFT is lipid soluble, peanut oil usually contained a high content of AFT. AFT is a class of highly toxic, mutagenic and carcinogenic compounds, which can severely damage human and animal liver tissue and lead to liver cancer. Therefore, the research of AFT removal method is of great significance. In recent years, physical adsorption has been widely used in vegetable oil industry because of its advantages of simplicity, convenience and quickness. However, the problem associated with adsorption is that it is difficult to separate from the oil system, which can lead to a heavy loss of oil. In order to remove AFT more effectively, magnetic multi-walled carbon nanotubes (mMWCNTs) were synthesized, and the effects of mMWCNTs on the removal effects of AFT in peanut oil were investigated under different adsorption conditions (adsorption time, adsorption temperature, the amount of adsorbent added, the initial content of AFT, and the magnetic load of adsorbent). The results showed that: when the adsorption time was 60 min, the adsorption temperature was 60 ℃ and the amount of adsorbent was 1.5 mg/g, mMWCNTs performed the best effect on AFT removal in peanut oil, and AFT removal rates were 92.36% and 90.28%, respectively. The content of AFT decreased from 100 ng/g to 20 ng/g. The results showed that the optimum adsorption conditions were as follows: adsorption time 60 min, adsorption temperature 60 ℃, adsorbent dosage 1.5 mg/g. The adsorbent had good removal effect, the operation was simple and rapid, and was suitable for the removal of aflatoxin from peanut oil.