Abstract: As one of the important raw materials of China′s food industry and feed industry, corn is rich in nutrients. Improper storage is easy to cause rapid growth and reproduction of microorganisms, which directly threatens storage safety. Therefore, it is urgent to explore an efficient, green and environmentally friendly method to inhibit the growth and reproduction of microorganisms during corn storage. Ozone can be used for killing microorganisms in grain storage due to its pollution-free, low cost, high sterilization efficiency, broad-spectrum and other characteristics. In this work, the sterilization rate was set as the response index, the appropriate level of different ozone treatment conditions was determined through single factor test, and the optimal sterilization process conditions of ozone treated corn were optimized by response surface method. Meanwhile, the changes of main quality indexes of corn before and after ozone treatment were monitored. The results showed that the factors affecting ozone sterilization rate were ozone treatment capacity, corn treatment capacity and ozone treatment time. The interaction between ozone treatment time and corn treatment capacity was significant (P<0.01). Based on the response surface test results of ozone sterilization rate and regression equation analysis, the mathematical model of the relationship between the sterilization rate and ozone treatment capacity, ozone treatment time, corn treatment capacity was established. The analysis showed that the determination coefficient R² of the regression equation was 0.992 4, indicating that the equation was significant and the response surface model fitted well, and the optimized sterilization process parameters obtained could be used for the sterilization process research of ozone treated corn. The optimized sterilization conditions of ozone treatment on corn were as follows: ozone treatment capacity 188.32 mg/m₃, ozone treatment time 24 min and corn treatment capacity 467 g, and under this condition, the sterilization rate of ozone was 82.28%, and the comparative study before and after ozone treatment showed that the optimized ozone sterilization process had no significant effect on the main quality indexes of corn. Ozone is a green and safe technology for grain storage and mildew prevention. The results of this study can provide a data basis for the safe storage of corn and the real warehouse application of ozone.