Abstract: Large yellow croaker roe phospholipid (LYCRPLs) is rich in polyunsaturated fatty acids such as DHA and EPA, and has high emulsification properties. To broaden the application value of LYCRPLs, the dispersions were prepared by employing a high-speed homogenization method with the utilization of LYCRPLs and Whey isolate protein (WPI). This study investigated the effect of pH, homogenization time, homogenization speed, WPI addition and LYCRPLs addition on the stability of LYCRPLs-WPI dispersions. On the basis of the single factor experiments, the particle size and clarification index of the dispersions were used as the response values, and the three factors with a greater influence on stability were selected to design the response surface analysis method. LYCRPLs-WPI dispersions were prepared under optimal process conditions and stored at temperature (25 ℃) for 28 d. The particle size, PDI and Zeta potential of the dispersions were measured every 7 d. The results showed that the influence factors on the stability of the dispersions were in this order: pH value＞LYCRPLs addition＞WPI addition＞homogenization speed＞homogenization time. Combined with the actual situation, the optimized condition for preparing the dispersions was as follows: 23 000 r/min homogenization speed, 1.0% WPI addition and 0.50% LYCRPLs addition, and under this condition, the particle size of the prepared dispersion was (215.20±2.12) nm and the clarification index was 0.080±0.003 as measured by three replicate experiments, which was close to the theoretical results, indicating that the model works well. Meanwhile, at the beginning of storage (0 d), the LYCRPLs-WPI dispersions were small in size, with a large absolute potential value and a more uniform distribution. In addition, the PDI values did not change significantly during the storage period. The results show excellent storage stability of the dispersion. These results provide a theoretical basis for the high value utilization LYCRPLs.