Abstract: To optimize the variable-temperature drying process of high-moisture wheat, reduce energy consumption and drying time, and enhance the post-drying quality, the effects of stage Ⅰ temperature (40-60 ℃), moisture conversion point (15%-19%), and stage Ⅱ temperature (45-65 ℃) on the drying rate, post-drying quality (color difference, hardness, sedimentation value, and seed vigor), as well as microstructural changes of high-moisture wheat were investigated. The results showed that the optimal variable-temperature drying parameters for high-moisture wheat were a stage Ⅰ variable-temperature of 46 ℃, a moisture conversion point 16%, and a stage Ⅱ temperature of 56 ℃. Under these conditions, the high-moisture wheat achieved a comprehensive score of 0.826, indicating good drying quality. The average error between the experimental and predicted values from the response surface methodology was 1.67%. This study provides a theoretical reference for the variable-temperature drying technology of high-moisture wheat.