5种多酚对小麦淀粉消化特性及面叶品质的影响

    Digestion characteristics of 5 kinds of polyphenols-containing wheat starches and their effects on noodle leaves quality

    • 摘要: 为了探究多酚对小麦淀粉消化特性和淀粉消化酶活性的影响,从原花青素、单宁酸、槲皮素、芦丁、儿茶素中选取效果较好的多酚添加到干面叶中,测定干面叶体外消化特性及各项品质指标。结果表明:5种多酚中单宁酸、原花青素对淀粉消化抑制效果较为显著,5.0%单宁酸和原花青素可使快速消化淀粉(RDS)显著降低至2.61%±0.44%、1.10%±0.22%,使抗性淀粉(RS)增加至91.17%±1.11%、87.82%±0.63%,增强了淀粉的抗消化性;5种多酚中单宁酸对α-淀粉酶抑制作用最强,其次为原花青素,当二者质量浓度为1.0 mg/mL时,抑制率分别为85.53%±2.21%、81.82%±2.02%;原花青素对α-葡萄糖苷酶抑制作用最强,当其质量浓度为3.0 mg/mL时,抑制率为78.27%±1.48%,而相同质量浓度的单宁酸对α-葡萄糖苷酶的抑制率仅为29.11%±1.07%;原花青素的添加可以使干面叶的硬度增大,蒸煮损失率先减小后增大,吸水率则与之相反,L*(亮度)显著减小,感官评分降低;当原花青素添加量为5.0%时,可使干面叶的血糖生成指数(GI)降低至68.47(中GI食物);原花青素在干面叶中的添加量不宜超过1.5%,否则会对干面叶的感官和食用品质产生不利影响。因此原花青素最佳添加量为1.0%,此时干面叶的GI可降低至77.08,且干面叶各项品质指标较好。本研究可为原花青素在低GI食品中的应用提供参考。

       

      Abstract: The study aimed to investigate the effects of polyphenols on wheat starch digestion characteristics and starch digestive enzyme activity, by selecting polyphenols with better from proanthocyanidins, tannic acid, quercetin, rutin, and catechin for incorporation into dried noodle leaves. Changes in the in vitro digestion characteristics and various quality indicators of the dried noodle leaves were then measured. The results showed that among the five polyphenols, tannic acid and proanthocyanidins had more significant inhibitory effects on starch digestion. With the addition of 5% tannic acid and proanthocyanidins, rapidly digestible starch (RDS) was significantly reduced to 2.61%±0.44% and 1.10%±0.22%, respectively, while resistant starch (RS) increased to 91.17%±1.11% and 87.82%±0.63%, respectively, enhancing the starch’s resistance to digestion. Among the five polyphenols, tannic acid exhibited the strongest inhibitory effect on α-amylase, with an inhibition rate of 85.53%±2.21% at a mass concentration of 1.0 mg·mL-1, followed by proanthocyanidins, which had an inhibition rate of 81.82%±2.02% at the same mass concentration. Proanthocyanidins demonstrated the strongest inhibitory effect on α-glucosidase, with an inhibition rate of 78.27%±1.48% at a mass concentration of 3.0 mg·mL-1, with tannic acid at the same mass concentration showed a much lower inhibition rate of 29.11%±1.07% against α-glucosidase, indicating poor effectiveness. Therefore, proanthocyanidins were selected for follow-up experiments. The addition of proanthocyanidins increased the hardness of the dried noodle leaves, with the cooking loss first increasing and then decreasing, while the water absorption rate showed an opposite trend. The L* value (brightness) decreased significantly, and the sensory scores were reduced. When the addition level was 5.0%, the glycemic index (GI) of dried noodle leaves was reduced to 68.47 (medium GI food), suggesting potential applications of proanthocyanidins in low-GI staple foods of diabetics. However, the addition should not exceed 1.5%, as equal to or exceeding this level. Thus, the optimal addition level of proanthocyanidins was determined to be 1.0%, at which the GI of the dried noodle leaves could be reduced to 77.08, and the dried noodle leaves exhibited good quality indicators. This provides a reference for the application of proanthocyanidins in low-GI foods.

       

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