Abstract: To solve the problems of low salbutamol residue concentrations and severe matrix interference in various livestock meat foods, an analytical method has been developed for the determination of salbutamol in livestock meat food using a novel electromembrane extraction (EME) technique combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were extracted using acetonitrile, followed by selective separation, purification, and enrichment using EME. Salbutamol detection was performed in positive ion mode with multiple reaction monitoring on an LC-MS/MS instrument, and quantification was conducted using the external standard method. In EME, salbutamol was first converted into its ionic form by adjusting the pH of the solution. Two L-shaped platinum electrodes were put into the sample solution and acceptor solution as the cathode and anode, respectively. The other ends of both L-shaped platinum electrodes were connected to the electrophoresis power supply. Upon activation of the power supply and stirring device, salbutamol in the sample phase migrate directionally through supported liquid membrane (SLM) into the acceptor phase. The results indicated that optimal extraction recovery of salbutamol was achieved under the conditions of an applied voltage of 80 V, a mixed solvent with ratio of di-(2-ethylhexyl) phosphate to 2-nitrophenyl octyl ether of 2:8 as the organic solvent, 20 mmol/L formic acid solution as the acceptor phase, and an extraction time of 30 min. This method exhibited a wide linear range of 1-1000 ng/mL with a correlation coefficient (R²) > 0.9996. The limits of detection (LOD, S/N=3) and the limits of quantification (LOQ, S/N=10) were 0.05 µg/kg and 0.17 µg/kg, respectively. Spike recovery experiments conducted on four different samples-beef, lamb, pork, and pork liver-yielded average recoveries ranging from 83.5% to 110% with relative standard deviations (RSDs, n=6) of 3.3% to 9.8%. This method is characterized by strong sample purification capabilities, rapid mass transfer rates, high sensitivity, and simple operation, making it suitable for highly sensitive and accurate determination of salbutamol in various livestock meat food.