Abstract: In order to screen out lactic acid bacteria strains with high β-glucosidase and tannase production, 34 lactic acid bacteria strains with strong growth and β-glucosidase production capacity were initially screened from 70 lactic acid bacteria strains derived from fermented foods by using the p-NPG method, and two strains with high tannase production were screened out by using the tannin-winding method and named GD03 and GD05, respectively, and further domestication of these two strains was carried out by using the concentration gradient method. The concentration gradient method was further used to domesticate the two lactic acid bacteria, and the intracellular and extracellular β-glucosidase and tannase extracellular enzyme activities of the strains were significantly increased after fermentation compared with those before fermentation (P < 0.05). The intracellular β-glucosidase enzyme activity increased 7-fold and 4-fold, the extracellular β-glucosidase enzyme activity increased 22-fold and the extracellular tannase enzyme activity increased 4-fold and 6-fold, respectively, after the fermentation of GD03 and GD05, respectively, compared with that before the fermentation. The soluble dietary fibre content increased 1-fold and 0.5-fold, and the tannin content decreased 50.69 % and 55.40 %, respectively. The results of this study provided a new idea for the screening of high-yielding β-glucosidase and tannase lactic acid bacteria, and laid a theoretical foundation for the application of high-yielding Lactobacillus in plant-based fermented foods.