具有潜在抗过敏活性乳酸菌的筛选及鉴定

    Screening and identification of lactic acid bacteria with potential anti-allergic activity

    • 摘要: 过敏性疾病是损害人体健康的常见疾病之一,严重危害患者的正常生活。为了筛选出具有潜在抗过敏的乳酸菌,通过MRS-CaCO3平板溶钙法和16S rDNA从传统发酵食品中分离鉴定乳酸菌,测定乳酸菌的益生特性,在此基础上,测定透明质酸酶抑制率,建立脂多糖诱导的RAW246.7细胞模型,测定菌株抗过敏潜能。研究结果表明:初步分离出5株乳酸菌,鉴定为Lactobacillus fermentum 2、Lactiplantibacillus plantarum 14、Latilactobacillus curvatus 27、Lactiplantibacillus plantarum 32和Lentilactobacillus parabuchneri 138;除了菌株27,其余菌株具有较强的产酸和生长能力;菌株14、138的模拟胃液耐受性、自聚集能力和对乙酸乙酯的疏水性均显著高于阳性对照菌株Lactobacillus rhamnosus GG(LGG)。所有菌株都有较高透明质酸酶抑制率(>70%),并且在脂多糖诱导的RAW246.7细胞中显著抑制一氧化氮(NO)的产生,说明它们具有潜在的抗过敏潜能;菌株都具有良好的抗生素敏感性,无溶血性,初步判定为安全菌株。该研究结果为抗过敏益生菌的筛选提供一定的理论依据,有助于菌种的开发和综合利用。

       

      Abstract: Allergic disease is a common disease that harms human health and seriously harms patients' normal life. To screen lactic acid bacteria with potential anti-allergic properties. Isolation and identification of lactic acid bacteria from traditional fermented foods by MRS-CaCO3 plate calcium solubilization and 16S rDNA. The probiotic properties of the strains were determined, including growth performance, acid production, gastrointestinal fluid tolerance, adhesion (co-aggregation, self-aggregation and hydrophobicity), and hyaluronidase inhibition. On this basis, the anti-allergic potential of the strains was determined by establishing a lipopolysaccharide-induced RAW246.7 cell model. The results of the study showed that five strains of lactic acid bacteria were initially isolated and identified as Lactobacillus fermentum 2, Lactiplantibacillus plantarum 14, Latilactobacillus curvatus 27, Lactiplantibacillus plantarum 32 and Lentilactobacillus parabuchneri 138. Except for strain 27, the remaining strains had a high capacity for acid production and growth. Strains 14 and 32 were highly acid-producing and could reach a pH of 3.88, while strain 138 had the strongest growth capacity. All strains showed high tolerance to simulated gastric fluid, with strains 14 and 138 being significantly more tolerant than the positive control Lactobacillus rhamnosus GG (LGG). Strain 14 has the best self-aggregating ability. Strain 14 and 138 had better hydrophobicity towards ethyl acetate, which was significantly higher than that of LGG. This indicates that the strain has good adhesion properties. All strains had similar co-aggregation ability for Escherichia coli and Staphylococcus aureus. Moreover, all strains had high hyaluronidase inhibition rates (> 70%) and significantly inhibited nitric oxide (NO) production in lipopolysaccharide-induced RAW246.7 cells, indicating their potential anti-allergic potential. In addition, the strains all have good antibiotic sensitivity and are strongly susceptible to ampicillin, erythromycin, chloramphenicol, and tetracycline. Hemolytic activity was not detected. In brief, Lactiplantibacillus plantarum 14 and Lentilactobacillus parabuchneri 138 have potential anti-allergic activity. The results of this study provide a certain theoretical basis for the screening of anti-allergic probiotics and help the development and comprehensive utilization of strains.

       

    /

    返回文章
    返回