负载茶多酚的酶解面筋蛋白-银耳多糖复合乳液对花生饮料品质的影响

    Effect of enzymatic hydrolysis gluten protein-Tremella fuciformis polysaccharide composite emulsion loaded with tea polyphenols on the quality of peanut beverage

    • 摘要: 为拓展面筋蛋白在乳液体系与植物蛋白饮料中的应用,同时提升花生饮料的营养功能、货架期稳定性及感官可接受度,以酶解面筋蛋白-银耳多糖复合物为复合壁材,构建负载茶多酚的W1/O/W2型双重乳液,并系统考察5%、10%、15%乳液添加量对花生蛋白饮料理化特性、抗氧化活性、贮藏稳定性及感官品质的影响。结果表明:在90 d贮藏期内,所有样品的pH值维持在6~7区间,色泽变化较小,菌落总数符合GB 4789.2—2022《食品安全国家标准》限值要求;贮藏90 d时,添加15%复合乳液的饮料表现出最佳抗氧化活性,其DPPH与ABTS自由基清除率分别达20.99%和20.79%,其离心沉淀率显著低于空白组,稳定系数均高于85.00%;饮料的表观黏度随剪切速率升高呈现典型剪切稀化行为,储能模量(G')与损耗模量(G'')均随角频率升高而增加,体系形成以弹性为主的弱凝胶网络结构,胶体稳定性显著提升;此外,复合乳液包埋体系能有效遮蔽茶多酚的苦涩味,显著改善饮料的感官可接受度。综上,所构建的负载茶多酚复合乳液可协同增强花生饮料的功能特性与物理稳定性,为面筋蛋白的高值化利用及功能性植物基饮品的开发提供理论支撑与技术参考。

       

      Abstract: To broaden the application of gluten protein in emulsion systems and plant protein beverages, and to simultaneously enhance the nutritional functionality, shelf-life stability, and sensory acceptability of peanut beverages, this study developed tea polyphenol-loaded W1/O/W2 double emulsions using an enzymatically hydrolyzed gluten protein-Tremella fuciformis polysaccharide complex as the composite wall material. The effects of 5%, 10%, and 15% emulsion incorporation levels on the physicochemical properties, antioxidant activity, storage stability, and sensory quality of peanut protein beverages were systematically evaluated. Results indicated that, over 90 days of storage, the pH range of all samples maintained 6~7, the color exhibited minor changes, and the total colony count complied with the limit requirements stipulated in GB 4789.2- 2022. After 90 days, the sample containing 15% composite emulsion demonstrated the highest antioxidant activity, with DPPH and ABTS radical scavenging rates of 20.99% and 20.79%, respectively. Its centrifugal precipitation rate was significantly lower than that of the control, and the stability coefficient was higher than 85.00%. All beverages displayed typical shear-thinning behavior; both the storage modulus (G') and loss modulus (G'') increased with angular frequency, forming an elasticity-dominated weak gel network that significantly enhanced colloidal stability. Furthermore, the emulsion system effectively masked the bitterness of tea polyphenols and improved sensory acceptability. In conclusion, the tea polyphenol-loaded composite emulsion synergistically enhanced both the functional and physical stability of peanut beverages, providing theoretical support and technical references for the high-value utilization of wheat gluten and the development of functional plant-based beverages.

       

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