Abstract: The enhanced chemiluminescence enzyme-linked immunoassay (ECLEIA) method was established for the determination of deoxynivalenol (DON) in the present study.The indirect competitive ECLEIA method was developed to examine DON using luminol-H₂O₂-HRP-4-Phenylphenol system under the optimal conditions,and was compared with ELISAand HPLC method.The linear regression equation ofECLEIAmethod was Y=-1 059.1 X+40 892 in the range of 0.01 to 1 000 μg/mL,while the correlation coefficient and limit of detection were 0.9 942 and 0.19 ng/mL,respectively.The RSD of mid-plate (n=3),intra-assay (n=3) and inter-assay (n=3) were 1.8% to 4.5%,2.4% to 7.4% and 7.8% to 11.0%,respectively.The recovery rates of ECLEIA in the wheat and corn samples with three concentration levels were from 90.0% to 126.8% and 97.7% to 110.0%,respectively.ECLEIA method was also used to determine the cross reaction rate of zearalenone,which also belonged to Fusarium toxin,less than 10%,and the mycotoxin of other species had almost no cross reaction.The established chemiluminescence enzyme-linked immunoassay (ECLEIA) method had the advantages of simple operation,high sensitivity and high specificity,which could be applied for the rapid detection of DON in the actual samples.