Abstract: In order to promote the formation of active components of Malus domeri (Bois) Chev. enzyme, the influences of yeast inoculation amount, acetic acid bacteria inoculation amount, lactic acid bacteria inoculation amount and fermentation temperature on total phenols mass concentration and superoxide dismutase (SOD) activity were explored through single factor and orthogonal experiments. The results indicated that the optimal fermentation conditions were as follows: yeast inoculation amount 1.0%, acetic acid bacteria inoculation amount 1.5%, lactic acid bacteria inoculation amount 1.0% and fermentation temperature 30 ℃. Under the optimal conditions, the total phenols mass concentration and SOD activity obtained by multi-strain fermentation were both higher than that of natural fermentation. The total phenol mass concentration reached the maximum 8.18 mg/mL, and the SOD activity was 17.71 U/mL at 21 d, which increased by 27.2% and 35.6% respectively. This research provides references for the further development and utilization of Malus domeri (Bois) Chev. enzyme.