4种茶的多酚含量、抗氧化性及抑制α-淀粉酶活性的比较

    Polyphenol Content,Antioxidative and Anti-α-amylase Activities of Four Tea Extracts

    • 摘要: 将新会柑普茶与普洱茶、毛尖、滇红几种常见的茶叶品种进行成分和功效比较,为这一茶叶家族新成员的健康功效提供科学依据,为其产业发展提供科学指导。以没食子酸为标准品,运用福林酚比色法比较柑普、普洱、滇红和毛尖的多酚含量;采用分光光度法测试4种茶的DPPH和ABTS自由基清除能力,从而评价4种茶的体外抗氧化活性;以阿卡波糖为阳性对照,采用分光光度法测试了茶乙醇粗提物对α-淀粉酶的抑制效果,初步评价4种茶的降糖活性。结果表明:4种茶提取物的多酚含量从高到低依次为毛尖(369.5 mg/g)、滇红(237.0 mg/g)、普洱(200.5 mg/g)、柑普(194.8 mg/g);4种茶提取物清除DPPH自由基的能力从强到弱依次是毛尖(IC50=4 μg/mL)、滇红(IC50=6μg/mL)、普洱(IC50=15μg/mL)和柑普(IC50=18μg/mL);4种茶提取物清除ABTS自由基的能力从强到弱依次为毛尖(IC50=2μg/mL)、滇红(IC50=3μg/mL)、柑普茶和普洱茶(IC50=7μg/mL);以阿卡波糖为阳性对照,4种茶对α-淀粉酶的抑制作用从强到弱依次为毛尖(IC50=12.9 mg/mL)、滇红(IC50=19.0 mg/mL)、普洱(IC50=21.0 mg/mL)和柑普(IC50=24.9 mg/mL),α-淀粉酶抑制能力均优于阿卡波糖(IC50=26.7 mg/mL)。4种茶均具有较好的抗氧化和α-淀粉酶抑制活性,且这两种生理活性与多酚含量呈正相关,在保健食品开发方面具有较大的潜力。

       

      Abstract: In order to provide scientific basis for the health benefits and guidance for the industrial development of Xinhui Ganpu tea,the composition and biologic function of Xinhui Ganpu tea with Pu'er,Maojian,and Dianhong were carried out in this study.The polyphenol contents were determined by folin-phenol colorimetry method using gallic acid as a reference.The DPPH and ABTS radical scavenging activities of ethanol extracts of four kinds of tea were determined by spectrophotometry method,evaluating the antioxidant activity of the four samples in vitro.Using acarbose as a positive control,the inhibitory effect of tea ethanol extract on the activity ofα-amylase was measured by spectrophotometry,and the hypoglycemic activity of four teas was evaluated initially.Results showed that the polyphenol contents of four samples from the highest to the lowest were Maojian(369.5mg/g),Dianhong(237.0 mg/g),Pu'er(200.5 mg/g) and Ganpu(194.8 mg/g).The DPPH radicals scavenging activities of four samples from the strongest to the weakest were Maojian(IC50=4μg/mL),Dianhong(IC50=6μg/mL),Pu'er(IC50=15μg/mL) and Ganpu(IC50=18μg/mL).The ABTS radicals scavenging activities of four samples from the strongest to the weakest were Maojian(IC50=2μg/mL),Dianhong(IC50==3μg/mL),Pu'er and Ganpu(IC50=7μg/mL).Using acarbose as positive control,four samples had obvious effect on inhibitingα-amylase,and they were Maojian(IC50=12.9 mg/mL),Dianhong(IC50=19.0 mg/mL),Pu'er(IC50=21.0 mg/mL) and Ganpu(IC50=24.9 mg/mL).Four samples have good anti-oxidative andα-amylase inhibitory activities,and the two physiological activities are positively related to polyphenol content.Four samples have great potential in the research and development of functional food.

       

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