Abstract: Fruits and vegetables are rich in nutrients and contain various ingredients,which are beneficial to human health.Flavonoids are bioactive substances and they have several biological activities such as antibiosis,anti-inflammatory,antioxidant,anti-tumor,anti-cancer,anti-virus activities and can also prevent and treat cardiovascular disease,etc.Flavonoids,as natural active ingredients,are safer in reducing the risk of disease compared with synthetic substances for the prevention and treatment of human diseases.Flavonoids are difficult to extract and detect due to the diversity of fruit and vegetable species,the complexity of nutrient matrix and the low amount of flavonoids.In recent years,researchers have paid extensive attention to the extraction methods of flavonoids with low cost,low pollution,high extraction rate and high efficiency,as well as the detection methods with simple and rapid operation,high accuracy and stable quality.The physiological function,optimized extraction technology,detection techniques and application of flavonoids in some fruits and vegetables were introduced in detail in this paper,aiming to provide the basic theoretical foundation for the economic development and comprehensive utilization of flavonoids in fruits and vegetables.