Abstract: The present study was to determine and analysis the major components of kenaf seeds and qualities and trace components of kenaf seeds oils from different cultivars.The oils were extracted from ground kenaf seed by solvent extraction method.The results showed that the crude oil contents of the four cultivars were ranging from 18.52% to 21.19%,and the average content was 19.83%;the protein contents were ranging from 25.37% to 29.57%,and the average content was 26.63%.Smaller differences were founded in the refractive index,relative density,saponification value and iodine value of kenaf seed oils from four different varieties;the acid values of kenaf seed oils were ranging from 1.09 mg/g to 16.72 mg/g,while the peroxide values were from 0.95 mmol/kg to 2.01 mmol/kg;the main compositions of fatty acids were linoleic acid (42.84%~50.79%),oleic acid (27.58%~32.38%),palmitic acid (17.04%~24.25%),stearic acid (1.93~2.61%) and linolenic acid (0.46%~0.61%).The vitamin E amounts of the kenaf seed oils were ranging from 85.81 mg/100g to 99.10 mg/100 g,and the average content was 93.30 mg/100 g;the main forms of vitamin E were α-tocopherols (18.41~26.69 mg/100 g) and γ-tocopherols (25.20~28.84 mg/100 g),which were accounted from 50.82% to 56.03% of the total vitamin E.The sterol contents of the four kenaf seed oils were ranging from 199.15 mg/100 g to 298.35 mg/100 g,and the average content was 242.42 mg/100 g;the compositions of sterols of kenaf seed oils from different varieties had significant difference,and β-sitosterol was indicated as the main component,which was accounted from 76.36% to 82.69%.The conspicuous differences were founded in β-carotene,phospholipid,and total phenol content of kenaf seed oils from different varieties,which were in the ranges of 0~9.66 mg/kg,1.68~4.53 mg/g and 5.78~23.22 mg/100 g,respectively.The above results implied that kenaf seed oil had the feasibility to be used as one kind of nutritious edible oil.The present study will provide theoretical basis for the cultivar breeding of kenaf and the development and utilization of kenaf seed oil.