漆黄素与环糊精的相互作用研究

毛善勇; 李向阳; 李刚

漆黄素与环糊精的相互作用研究

1. 广州金至检测技术有限公司, 广东广州 510330;
2. 新乡职业技术学院, 河南新乡 453006;
3. 河南科技学院, 河南新乡 453003

基金项目:

河南省高等学校重点科研项目（19A550009）

详细信息

作者简介:
毛善勇(1978-),男,浙江江山人,硕士,研究方向为食品、药品、日化产品分析与检测

通讯作者:
李刚,讲师,E-mail:lg760925@163.com

中图分类号: TS201.2
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出版历程
- 收稿日期: 2019-01-28
- 网络出版日期: 2022-09-27

Interaction between Fisetin and Cyclodextrins

1. Guangzhou King Test technology Co., Ltd., Guangzhou 510330, China;
2. Xinxiang Vocational and Technical College, Xinxiang 453006, China;
3. Henan Institute of Science and Technology, Xinxiang 453003, China

摘要

摘要: 漆黄素具有抗氧化、降肿瘤等多种生物活性功能，但其在水中溶性低、稳定性差的缺点制约了它在医药、化妆品和食品中的应用，而采用环糊精包埋是一条可行之路。采用相溶解度法比较了α-环糊精（α-CD）、β-环糊精（β-CD）、γ-环糊精（γ-CD）和麦芽糖-β-环糊精（M-β-CD）对漆黄素的包埋效果。在此基础上制备了漆黄素与M-β-CD的复合物，运用紫外（UV）、红外（IR）及X射线衍射（XRD）方法对其结构进行了表征，并采用分子对接法建立了漆黄素与环糊精复合物的三维超分子结构。结果表明：M-β-CD和β-CD对漆黄素的包合效果接近，显著优于α-CD和γ-CD。漆黄素与M-β-CD复合后，其物相发生了重大改变，分子对接分析表明，漆黄素沿M-β-CD的大口端方向进入其空腔形成复合物，该超分子结构可能通过氢键维持。
- 漆黄素 /
- 环糊精 /
- 复合物 /
- 相互作用 /
- 分子对接
Abstract: Fisetin possesses many health-promoting properties,such as antioxidant,anticancer activities.Its poor solubility and stability in water limit its application in medicines,cosmetics and foods.Its shortcomings can be overcome by complexing with cyclodextrins (CDs).But there is no systematic report about its interaction with CDs.In view of this,the effect of ɑ-cyclodextrin (α-CD),β-cyclodextrin (β-CD),γ-cyclodextrin (γ-CD) and maltosyl-β-cyclodextrin (M-β-CD) on the aqueous solubility of fisetin was compared by the phase solubility method.Then,the complex of fisetin and M-β-CD was prepared and characterized by using ultraviolet-visible spectrum (UV),ultraviolet-visible spectrum (IR) and X-ray diffraction (XRD).Based on the experimental results,the three-dimensional supermolecular structures of fisetin/CD complexes was established by using molecular docking method.It was found that the inclusion effects of M-β-CD and β-CD on fisetin were higher than those of α-CD and β-CD.After complexing with M-β-CD,the physicochemical properties of fisetin were significantly changed,which were completely dispersed in M-β-CD.The molecular docking analysis suggested that fisetin entered the hydrophobic cavity from the wide rim of M-β-CD,and the complex was maintained by hydrogen bonding.
- fisetin /
- cyclodextrin /
- complex /
- interaction /
- molecular docking

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参考文献(15)

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