WU Yousheng, WANG Le, ZHANG Mengyue, LIANG Ying, WANG Jinshui. Preparation of the immobilized laccase remover with modified montmorillonite for the removal of aflatoxin B1[J]. Journal of Henan University of Technology(Natural Science Edition), 2024, 45(4): 64-74. DOI: 10.16433/j.1673-2383.202312170001
    Citation: WU Yousheng, WANG Le, ZHANG Mengyue, LIANG Ying, WANG Jinshui. Preparation of the immobilized laccase remover with modified montmorillonite for the removal of aflatoxin B1[J]. Journal of Henan University of Technology(Natural Science Edition), 2024, 45(4): 64-74. DOI: 10.16433/j.1673-2383.202312170001

    Preparation of the immobilized laccase remover with modified montmorillonite for the removal of aflatoxin B1

    • In this study, the removal agent of modified montmorillonite immobilized laccase (MMT-CTAB-Lac) was prepared for the removal of Aflatoxin B1 (AFB1). After the treatment of cetyltrimethyl ammonium bromide (CTAB), the MMT-CTAB-Lac was prepared by the modified montmorillonite (MMT-CTAB) with the immobilized laccase. The adsorption kinetics of the removal agent was investigated by the equation fitting. The conditions for removing AFB1 from the MMT-CTAB-Lac were optimized. The AFB1 degradation pathway and the toxicity of its degradation products were investigated by the LC-MS and T.E.S.T software. After the modification, the remover had larger layer spacing and pore volume, smaller particle size, and the increased specific surface area for the AFB1 adsorption. The AFB1 adsorbed on the remover was degraded by the immobilized laccase, which effectively prevented the accumulation of AFB1 on the remover, and significantly increased the adsorption capacity of MMT-CTAB-Lac. The chemisorption was a rate-limiting step in the adsorption process of AFB1. The adsorption capacity of MMT-CTAB-Lac for AFB1 was 44.5 mg·g-1, which was higher than that of MMT-CTAB. The removal rate of AFB1 by the MMT-CTAB-Lac was 67%, with the high catalytic degradation rate under the weak acid and neutral condition. The degradation of AFB1 by MMT-CTAB-Lac was further identified. The two possible degradation pathways were proposed: the first possible degradation pathway (destruction of lactone ring structure), with the degradation products of C17H14O7 and C16H12O5; the second possible degradation pathway (destruction of furan ring structure), with the degradation products of C17H14O7, C16H12O7, and C14H10O4. Through the toxicity prediction of T.E.S.T software, the toxicities of degradation products were reduced. The MMT-CTAB-Lac developed in this study lays an important foundation for the safe and efficient removal of AFB1 in the field of grain, oil and food.
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