STUDY ON THE ETHANOL EXTRACTION OF CRUDE EGG YOLK PHOSPHOLIPIDS

The phospholipids in deoiled egg yolk powder was extracted from deoiled egg yolk powder using ethanol as extraction solvent.First,the ethanol concentration was selected,and then the effects of extraction time,material-liquid ratio,extraction temperature,stirring rate,and extraction times on the effect of ethanol extraction of crude egg yolk phospholipids were investigated through single-factor experiments.Next,the response surface experiments were designed to optimize the reaction conditions of the ethanol extraction of crude egg yolk phospholipids according to the Box-Behnken center combination experimental design principle,and the purity and extraction rate of phospholipids in the crude egg yolk phospholipids were the response values.The optimal reaction conditions were as follows:ethanol concentration 95%,extraction time 4 min,material-liquid ratio 1:5 g/mL,extraction temperature 65℃,stirring rate 168 r/min,and extraction twice.Based on the above parameters,the purity and extraction rate of phospholipids in the crude egg yolk phospholipids were 71.03% and 66.17%.