STUDY ON PREPARATION OF ANTIOXIDANT PEPTIDES WITH TWO ENZYMES HYDROLYZING SESAME 11S PROTEIN

protein,hydrolysis pH 8.6,substrate concentration 3%,time 1.96 h.Under this condition,the actual yield of polypeptide was (77.41+0.30)%,the total antioxidant activity was (1.40+0.02) mmol/L,the IC₅₀ value of DPPH radical scavenging rate was 1.41 mg/mL,and the IC₅₀ value of ABTS radical scavenging rate was 1.06 mg/mL.The results show that the regression model established by the response surface is accurate and reliable.Compared with other natural antioxidant peptides,sesame 11S protein showed better antioxidant activity and had the ability to prepare high-activity anti-oxidant peptides,which provided a reference for further isolation and purification of sesame 1 1S anti-oxidant peptides.