GLUTATHIONE BIOSENSOR BASED ON Fe₃O₄NANOPARTICLES COMPOSITE FOR DETECTION OF ACRYLAMIDE

Acrylamide (AA) is a kind of harmful substance which can produce neurotoxicity,reproductive development toxicity,genetic toxicity and carcinogenicity to human body.AA can be produced in high starch and protein food after high temperature treatment,so it is very important to establish a method for rapid detection of AA.The main metabolic way of AA in human body is that acrylamide (AA) reacted with reduced glutathione (GSH) or hemoglobin (Hb).GSH is easy to undergo redox-reaction in air,so Hb-modified biosensor is mostly used in the electrochemical detection of AA.Fe₃O₄nanoparticles have good biocompatibility and can be combined with the-COOH group in GSH,which does not affect the oxidation performance of GSH,and can improve the electrical signal of GSH.Nafion/GSH-Fe₃O₄/GC sensors were prepared and the AA in real samples was detected qualitatively and quantitatively by cyclic voltammetry in the present study.The optimum detection conditions of AA were as follows:pH of 4.5,the amount of modified GSH of 100 μg and reaction time of 60 s.Under the optimal conditions,the AA of different concentrations was detected to establish the standard curve.The linear response w as in the range of 1×10^-7~1×10^-5,the linear regression equation was Y=-0.299X+2.699(R²=0.9967),and the recovery rate of the sample was 100.45%.The rapid detection of acrylamide has been realized by Nafion/GSH-Fe₃O₄/GC sensors method,which will provide a theoretical basis for the electrochemical detection of AA.