Transcriptome Sequencing and Functional Annotation Analysis of Grain Stored Pests, Plodia Interpunctella(Hübener)

The Indian meal moth, Plodia interpunctella (Hübner) (Lepidoptera:Pyralidae) is a destructive polyphagous storage pest and causes infestations in a wide array of commodities.In order to establish a transcriptome database of the Indian meal moth, Illumina Hi Seq 2500 platform was used to sequence the transcriptome of P.interpunctella, and the raw sequence data reads were filtered and assembled, and bioinformatics analysis was also carried out.The results showed that 116 399 unigenes with the mean lengths of 1 079 bp was acquired.Through a similarity search against seven public databases, 63 799 unigenes were annotated.According to GO category, a total of 45, 225 unigenes divided into 55 subclass were mapped to three GO groups:biological process, cellular component, and molecular function;a total of 24 658 unigenes were classified to 26 KOG categories;34 202 unigenes were divided into five KEGG functional categories.The microsatellite(SSR) analysis indicated that a total of 3 101 SSR markers were detected.This study obtained the transcriptome data of P.interpunctella, which can provide a foundation for the further study of gene functions, and functional analysis of olfactory genes and development of molecular markers in P.interpunctella.